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u2os human bone sarcoma cell line  (ATCC)


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    ATCC u2os human bone sarcoma cell line
    A.Lollipop chart for subcellular location of the 67 versus 40 proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 (top) and I-BET151 (middle), respectively. Subcellular location of each protein, based on immunohistochemistry and confocal microscopy, was retrieved from the Human Protein Atlas ( proteinatlas.org ). When a protein had more than one subcellular location assigned, that protein was included once per subcellular location in the graph. Bottom graph show a Venn diagram of the overlap between proteins stabilized/destabilized in (+)-JQ1 and I-BET151. Only 11 proteins were found to overlap between the two compounds. B. Physical protein-protein interaction (PPI) networks for the proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 and I-BET151, respectively. PPI networks were retrieved from the STRING db. Nodes are colored by subcellular location (from Human Protein Atlas). Large nodes correspond to proteins detected by TPP, where small nodes are additionally added nodes from the STRING db during network retrieval. C. Top: Physical PPI networks for (+)-JQ1 (same as B), except green nodes here indicate proteins found to be stabilized by (+)-JQ1 in cell extract. Bottom: Venn diagram for overlap of proteins detected in whole cells versus cell extract after perturbation with (+)-JQ1. D. Radar chart for features in cell painting data for (+)-JQ1 and I-BET151. Features were grouped into categories based on two criteria: (i) Cell Profiler module, i.e. Intensity (I), Correlation (C), Granularity (G), Location (L) and RadialDistribution (RD); and (ii) stains, i.e. Nucleus (Hoechst), ER (Concanavalin A), Nucleoli and cytoplasmic RNA (SYTO14), Golgi apparatus and F-actin cytoskeleton (WGA and Phalloidin) and Mitochondria (Mitotracker). Features were only consider for the object Cell, except for features from the stain for Nucleus, which was only considered in the object Nucleus. Additionally, area-shape related features were grouped by cell compartment, i.e. Cell (C), Cytoplasm (Cy) and Nucleus (N). E. t-SNE for the morphological features in the SPECS cell painting data on <t>U2OS</t> cells. Blue dots show the location of all cells treated with BET bromodomain inhibitors sharing at least one target with either (+)-JQ1 or I-BET151. Despite several BET bromodomain inhibitors clustering close to (+)-JQ1 or I-BET151, there are also several compounds with distinctly different morphological changes.
    U2os Human Bone Sarcoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u2os human bone sarcoma cell line/product/ATCC
    Average 99 stars, based on 8316 article reviews
    u2os human bone sarcoma cell line - by Bioz Stars, 2026-05
    99/100 stars

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    1) Product Images from "Integrating Cell Painting and Thermal Proteome Profiling for Inference of Targets and Mechanism of Action"

    Article Title: Integrating Cell Painting and Thermal Proteome Profiling for Inference of Targets and Mechanism of Action

    Journal: bioRxiv

    doi: 10.1101/2025.05.30.657006

    A.Lollipop chart for subcellular location of the 67 versus 40 proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 (top) and I-BET151 (middle), respectively. Subcellular location of each protein, based on immunohistochemistry and confocal microscopy, was retrieved from the Human Protein Atlas ( proteinatlas.org ). When a protein had more than one subcellular location assigned, that protein was included once per subcellular location in the graph. Bottom graph show a Venn diagram of the overlap between proteins stabilized/destabilized in (+)-JQ1 and I-BET151. Only 11 proteins were found to overlap between the two compounds. B. Physical protein-protein interaction (PPI) networks for the proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 and I-BET151, respectively. PPI networks were retrieved from the STRING db. Nodes are colored by subcellular location (from Human Protein Atlas). Large nodes correspond to proteins detected by TPP, where small nodes are additionally added nodes from the STRING db during network retrieval. C. Top: Physical PPI networks for (+)-JQ1 (same as B), except green nodes here indicate proteins found to be stabilized by (+)-JQ1 in cell extract. Bottom: Venn diagram for overlap of proteins detected in whole cells versus cell extract after perturbation with (+)-JQ1. D. Radar chart for features in cell painting data for (+)-JQ1 and I-BET151. Features were grouped into categories based on two criteria: (i) Cell Profiler module, i.e. Intensity (I), Correlation (C), Granularity (G), Location (L) and RadialDistribution (RD); and (ii) stains, i.e. Nucleus (Hoechst), ER (Concanavalin A), Nucleoli and cytoplasmic RNA (SYTO14), Golgi apparatus and F-actin cytoskeleton (WGA and Phalloidin) and Mitochondria (Mitotracker). Features were only consider for the object Cell, except for features from the stain for Nucleus, which was only considered in the object Nucleus. Additionally, area-shape related features were grouped by cell compartment, i.e. Cell (C), Cytoplasm (Cy) and Nucleus (N). E. t-SNE for the morphological features in the SPECS cell painting data on U2OS cells. Blue dots show the location of all cells treated with BET bromodomain inhibitors sharing at least one target with either (+)-JQ1 or I-BET151. Despite several BET bromodomain inhibitors clustering close to (+)-JQ1 or I-BET151, there are also several compounds with distinctly different morphological changes.
    Figure Legend Snippet: A.Lollipop chart for subcellular location of the 67 versus 40 proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 (top) and I-BET151 (middle), respectively. Subcellular location of each protein, based on immunohistochemistry and confocal microscopy, was retrieved from the Human Protein Atlas ( proteinatlas.org ). When a protein had more than one subcellular location assigned, that protein was included once per subcellular location in the graph. Bottom graph show a Venn diagram of the overlap between proteins stabilized/destabilized in (+)-JQ1 and I-BET151. Only 11 proteins were found to overlap between the two compounds. B. Physical protein-protein interaction (PPI) networks for the proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 and I-BET151, respectively. PPI networks were retrieved from the STRING db. Nodes are colored by subcellular location (from Human Protein Atlas). Large nodes correspond to proteins detected by TPP, where small nodes are additionally added nodes from the STRING db during network retrieval. C. Top: Physical PPI networks for (+)-JQ1 (same as B), except green nodes here indicate proteins found to be stabilized by (+)-JQ1 in cell extract. Bottom: Venn diagram for overlap of proteins detected in whole cells versus cell extract after perturbation with (+)-JQ1. D. Radar chart for features in cell painting data for (+)-JQ1 and I-BET151. Features were grouped into categories based on two criteria: (i) Cell Profiler module, i.e. Intensity (I), Correlation (C), Granularity (G), Location (L) and RadialDistribution (RD); and (ii) stains, i.e. Nucleus (Hoechst), ER (Concanavalin A), Nucleoli and cytoplasmic RNA (SYTO14), Golgi apparatus and F-actin cytoskeleton (WGA and Phalloidin) and Mitochondria (Mitotracker). Features were only consider for the object Cell, except for features from the stain for Nucleus, which was only considered in the object Nucleus. Additionally, area-shape related features were grouped by cell compartment, i.e. Cell (C), Cytoplasm (Cy) and Nucleus (N). E. t-SNE for the morphological features in the SPECS cell painting data on U2OS cells. Blue dots show the location of all cells treated with BET bromodomain inhibitors sharing at least one target with either (+)-JQ1 or I-BET151. Despite several BET bromodomain inhibitors clustering close to (+)-JQ1 or I-BET151, there are also several compounds with distinctly different morphological changes.

    Techniques Used: Immunohistochemistry, Confocal Microscopy, Staining



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    u2os human bone sarcoma cell line  (ATCC)
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    ATCC u2os human bone sarcoma cell line
    A.Lollipop chart for subcellular location of the 67 versus 40 proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 (top) and I-BET151 (middle), respectively. Subcellular location of each protein, based on immunohistochemistry and confocal microscopy, was retrieved from the Human Protein Atlas ( proteinatlas.org ). When a protein had more than one subcellular location assigned, that protein was included once per subcellular location in the graph. Bottom graph show a Venn diagram of the overlap between proteins stabilized/destabilized in (+)-JQ1 and I-BET151. Only 11 proteins were found to overlap between the two compounds. B. Physical protein-protein interaction (PPI) networks for the proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 and I-BET151, respectively. PPI networks were retrieved from the STRING db. Nodes are colored by subcellular location (from Human Protein Atlas). Large nodes correspond to proteins detected by TPP, where small nodes are additionally added nodes from the STRING db during network retrieval. C. Top: Physical PPI networks for (+)-JQ1 (same as B), except green nodes here indicate proteins found to be stabilized by (+)-JQ1 in cell extract. Bottom: Venn diagram for overlap of proteins detected in whole cells versus cell extract after perturbation with (+)-JQ1. D. Radar chart for features in cell painting data for (+)-JQ1 and I-BET151. Features were grouped into categories based on two criteria: (i) Cell Profiler module, i.e. Intensity (I), Correlation (C), Granularity (G), Location (L) and RadialDistribution (RD); and (ii) stains, i.e. Nucleus (Hoechst), ER (Concanavalin A), Nucleoli and cytoplasmic RNA (SYTO14), Golgi apparatus and F-actin cytoskeleton (WGA and Phalloidin) and Mitochondria (Mitotracker). Features were only consider for the object Cell, except for features from the stain for Nucleus, which was only considered in the object Nucleus. Additionally, area-shape related features were grouped by cell compartment, i.e. Cell (C), Cytoplasm (Cy) and Nucleus (N). E. t-SNE for the morphological features in the SPECS cell painting data on <t>U2OS</t> cells. Blue dots show the location of all cells treated with BET bromodomain inhibitors sharing at least one target with either (+)-JQ1 or I-BET151. Despite several BET bromodomain inhibitors clustering close to (+)-JQ1 or I-BET151, there are also several compounds with distinctly different morphological changes.
    U2os Human Bone Sarcoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    99/100 stars
    		  Buy from Supplier
    u2os human bone sarcoma htb 96 cell lines  (ATCC)
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    ATCC u2os human bone sarcoma htb 96 cell lines
    A.Lollipop chart for subcellular location of the 67 versus 40 proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 (top) and I-BET151 (middle), respectively. Subcellular location of each protein, based on immunohistochemistry and confocal microscopy, was retrieved from the Human Protein Atlas ( proteinatlas.org ). When a protein had more than one subcellular location assigned, that protein was included once per subcellular location in the graph. Bottom graph show a Venn diagram of the overlap between proteins stabilized/destabilized in (+)-JQ1 and I-BET151. Only 11 proteins were found to overlap between the two compounds. B. Physical protein-protein interaction (PPI) networks for the proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 and I-BET151, respectively. PPI networks were retrieved from the STRING db. Nodes are colored by subcellular location (from Human Protein Atlas). Large nodes correspond to proteins detected by TPP, where small nodes are additionally added nodes from the STRING db during network retrieval. C. Top: Physical PPI networks for (+)-JQ1 (same as B), except green nodes here indicate proteins found to be stabilized by (+)-JQ1 in cell extract. Bottom: Venn diagram for overlap of proteins detected in whole cells versus cell extract after perturbation with (+)-JQ1. D. Radar chart for features in cell painting data for (+)-JQ1 and I-BET151. Features were grouped into categories based on two criteria: (i) Cell Profiler module, i.e. Intensity (I), Correlation (C), Granularity (G), Location (L) and RadialDistribution (RD); and (ii) stains, i.e. Nucleus (Hoechst), ER (Concanavalin A), Nucleoli and cytoplasmic RNA (SYTO14), Golgi apparatus and F-actin cytoskeleton (WGA and Phalloidin) and Mitochondria (Mitotracker). Features were only consider for the object Cell, except for features from the stain for Nucleus, which was only considered in the object Nucleus. Additionally, area-shape related features were grouped by cell compartment, i.e. Cell (C), Cytoplasm (Cy) and Nucleus (N). E. t-SNE for the morphological features in the SPECS cell painting data on <t>U2OS</t> cells. Blue dots show the location of all cells treated with BET bromodomain inhibitors sharing at least one target with either (+)-JQ1 or I-BET151. Despite several BET bromodomain inhibitors clustering close to (+)-JQ1 or I-BET151, there are also several compounds with distinctly different morphological changes.
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    https://www.bioz.com/result/u2os human bone sarcoma htb 96 cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
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    Image Search Results


    A.Lollipop chart for subcellular location of the 67 versus 40 proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 (top) and I-BET151 (middle), respectively. Subcellular location of each protein, based on immunohistochemistry and confocal microscopy, was retrieved from the Human Protein Atlas ( proteinatlas.org ). When a protein had more than one subcellular location assigned, that protein was included once per subcellular location in the graph. Bottom graph show a Venn diagram of the overlap between proteins stabilized/destabilized in (+)-JQ1 and I-BET151. Only 11 proteins were found to overlap between the two compounds. B. Physical protein-protein interaction (PPI) networks for the proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 and I-BET151, respectively. PPI networks were retrieved from the STRING db. Nodes are colored by subcellular location (from Human Protein Atlas). Large nodes correspond to proteins detected by TPP, where small nodes are additionally added nodes from the STRING db during network retrieval. C. Top: Physical PPI networks for (+)-JQ1 (same as B), except green nodes here indicate proteins found to be stabilized by (+)-JQ1 in cell extract. Bottom: Venn diagram for overlap of proteins detected in whole cells versus cell extract after perturbation with (+)-JQ1. D. Radar chart for features in cell painting data for (+)-JQ1 and I-BET151. Features were grouped into categories based on two criteria: (i) Cell Profiler module, i.e. Intensity (I), Correlation (C), Granularity (G), Location (L) and RadialDistribution (RD); and (ii) stains, i.e. Nucleus (Hoechst), ER (Concanavalin A), Nucleoli and cytoplasmic RNA (SYTO14), Golgi apparatus and F-actin cytoskeleton (WGA and Phalloidin) and Mitochondria (Mitotracker). Features were only consider for the object Cell, except for features from the stain for Nucleus, which was only considered in the object Nucleus. Additionally, area-shape related features were grouped by cell compartment, i.e. Cell (C), Cytoplasm (Cy) and Nucleus (N). E. t-SNE for the morphological features in the SPECS cell painting data on U2OS cells. Blue dots show the location of all cells treated with BET bromodomain inhibitors sharing at least one target with either (+)-JQ1 or I-BET151. Despite several BET bromodomain inhibitors clustering close to (+)-JQ1 or I-BET151, there are also several compounds with distinctly different morphological changes.

    Journal: bioRxiv

    Article Title: Integrating Cell Painting and Thermal Proteome Profiling for Inference of Targets and Mechanism of Action

    doi: 10.1101/2025.05.30.657006

    Figure Lengend Snippet: A.Lollipop chart for subcellular location of the 67 versus 40 proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 (top) and I-BET151 (middle), respectively. Subcellular location of each protein, based on immunohistochemistry and confocal microscopy, was retrieved from the Human Protein Atlas ( proteinatlas.org ). When a protein had more than one subcellular location assigned, that protein was included once per subcellular location in the graph. Bottom graph show a Venn diagram of the overlap between proteins stabilized/destabilized in (+)-JQ1 and I-BET151. Only 11 proteins were found to overlap between the two compounds. B. Physical protein-protein interaction (PPI) networks for the proteins found to be either stabilized or destabilized in TPP for compounds (+)-JQ1 and I-BET151, respectively. PPI networks were retrieved from the STRING db. Nodes are colored by subcellular location (from Human Protein Atlas). Large nodes correspond to proteins detected by TPP, where small nodes are additionally added nodes from the STRING db during network retrieval. C. Top: Physical PPI networks for (+)-JQ1 (same as B), except green nodes here indicate proteins found to be stabilized by (+)-JQ1 in cell extract. Bottom: Venn diagram for overlap of proteins detected in whole cells versus cell extract after perturbation with (+)-JQ1. D. Radar chart for features in cell painting data for (+)-JQ1 and I-BET151. Features were grouped into categories based on two criteria: (i) Cell Profiler module, i.e. Intensity (I), Correlation (C), Granularity (G), Location (L) and RadialDistribution (RD); and (ii) stains, i.e. Nucleus (Hoechst), ER (Concanavalin A), Nucleoli and cytoplasmic RNA (SYTO14), Golgi apparatus and F-actin cytoskeleton (WGA and Phalloidin) and Mitochondria (Mitotracker). Features were only consider for the object Cell, except for features from the stain for Nucleus, which was only considered in the object Nucleus. Additionally, area-shape related features were grouped by cell compartment, i.e. Cell (C), Cytoplasm (Cy) and Nucleus (N). E. t-SNE for the morphological features in the SPECS cell painting data on U2OS cells. Blue dots show the location of all cells treated with BET bromodomain inhibitors sharing at least one target with either (+)-JQ1 or I-BET151. Despite several BET bromodomain inhibitors clustering close to (+)-JQ1 or I-BET151, there are also several compounds with distinctly different morphological changes.

    Article Snippet: For the purpose of this study, U2OS human bone sarcoma cell line (sourced from ATCC U2OS #HTB-96) was expanded and painted at passage number +8.

    Techniques: Immunohistochemistry, Confocal Microscopy, Staining